Journal: Nature protocols

Article Title: O 2 -controllable hydrogels for studying cellular responses to hypoxic gradients in three dimensions in vitro and in vivo

doi: 10.1038/nprot.2017.059

Figure Lengend Snippet: Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors (PreSens) were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.

Article Snippet: Microx TX3 microfiber-optic oxygen transmitter; temperature-compensated system (PreSens) Needle-type oxygen microsensors (PreSens) Manual micromanipulator (PreSens) 3D real-time imaging Confocal microscope (e.g., Carl Zeiss, model no. LSM 780) Cell tracking analysis Imaris (Bitplane, http://www.bitplane.com/download ) MATLAB (MathWorks, https://www.mathworks.com/products/matlab.html ) Prism (GraphPad, https://www.graphpad.com/scientific-software/prism/ ) Protease activity POLARstar OPTIMA plate reader (BMG Labtech) or similar fluorescent plate reader for wavelength measurements of fluorescent signals Cell proliferation assay UV/visible spectrometer (SpectraMax; Molecular Devices) RNA extraction NanoDrop spectrophotometer (Thermo Fisher Scientific, model no. ND-1000) Blood vessel perfusion Confocal microscope (Carl Zeiss, model no. LSM 780 Meta) General laboratory equipment Cell culture incubator Sterile 96-well plates for 3D cell culture Hemocytometer or cell counter Positive-displacement pipette and sterile capillary piston tips Water bath Heat block Sterile 1.5-, 2-, 15-, and 50-ml conical (micro)centrifuge tubes Glass beaker, 50 ml Spatula Forceps REAGENT SETUP !

Techniques: In Vivo

Journal: Micromachines

Article Title: A Resonant Pressure Microsensor with a Wide Pressure Measurement Range

doi: 10.3390/mi12040382

Figure Lengend Snippet: ( a ) The stress distribution on sensing elements under pressure; ( b ) the working principle of the resonator; ( c ) the working principle of the presented microsensor.

Article Snippet: To verify the design of the microsensor, FEA simulations based on ANSYS were employed to evaluate the maximum stress on the microsensors with two different pressure-sensitive diaphragms.

Techniques:

Journal: Micromachines

Article Title: A Resonant Pressure Microsensor with a Wide Pressure Measurement Range

doi: 10.3390/mi12040382

Figure Lengend Snippet: ( a ) Structural simulations under 7000 kPa: ( a ) the microsensor with square pressure-sensitive diaphragm (side length of 4.4 mm); ( b ) the microsensor with circular pressure-sensitive diaphragm (diameter of 4.4 mm).

Article Snippet: To verify the design of the microsensor, FEA simulations based on ANSYS were employed to evaluate the maximum stress on the microsensors with two different pressure-sensitive diaphragms.

Techniques:

Journal: Micromachines

Article Title: A Resonant Pressure Microsensor with a Wide Pressure Measurement Range

doi: 10.3390/mi12040382

Figure Lengend Snippet: Dimensional information of the microsensor model in FEA simulations.

Article Snippet: To verify the design of the microsensor, FEA simulations based on ANSYS were employed to evaluate the maximum stress on the microsensors with two different pressure-sensitive diaphragms.

Techniques:

Journal: Micromachines

Article Title: A Resonant Pressure Microsensor with a Wide Pressure Measurement Range

doi: 10.3390/mi12040382

Figure Lengend Snippet: The fabrication results: ( a ) top view of the “H” resonant beam; ( b ) an SEM cross-section image of the microsensor; ( c ) top view of the microsensor and ( d ) side view of the microsensor.

Article Snippet: To verify the design of the microsensor, FEA simulations based on ANSYS were employed to evaluate the maximum stress on the microsensors with two different pressure-sensitive diaphragms.

Techniques:

Journal: Micromachines

Article Title: A Resonant Pressure Microsensor with a Wide Pressure Measurement Range

doi: 10.3390/mi12040382

Figure Lengend Snippet: ( a ) The cross-section image of the resonant pressure sensor’s packaging concept; ( b ) an image of the packaged microsensor.

Article Snippet: To verify the design of the microsensor, FEA simulations based on ANSYS were employed to evaluate the maximum stress on the microsensors with two different pressure-sensitive diaphragms.

Techniques: